this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...

ninety barley doubled haploid lines originated from crossing between six barley cultivars in cereal research department, seed and plant improvement institute, karaj, iran were evaluated for resistance to powdery mildew and some agronomic traits in field in 2007. six parent cultivars and a powdery mildew susceptible check cultivar, afzal were also included. sixty two doubled haploid lines showed...

salinity tolerance is a genetically and physiologically complex trait, controlled by quantitative trait loci (qtls). in order to map the qtls associated with agronomic and physiological traits, 149 doubled haploid lines derived from a cross between clipper (salt susceptible) and sahara 3771 (salt tolerant) barley genotypes were evaluated under natural saline-stress and non-stress conditions usi...

background:  stresses such as heat shock, starvation, or osmotic is essential to lead isolated microspores towards embryogenesis. despite the effectiveness of stresses in embryogenesis, they exert adverse effects on metabolism and growth of the regenerated plants.objectives: the effects of heat shock and 2,4-d treatment on total protein content of treated microspores, morphological and physiolo...

in order to locate the qtls for plant height, peduncle length, and heading date, a set of 107 wheat doubled haploid (dh) lines derived from the cross fukuho-komugi× oligoculm was grown during the growing seasons of 2004 and 2005. a total of 36 qtls were identified based on composite interval mapping (cim) approach. all detected plant height qtl’s were stable over the two years. qtls located nea...

Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 ...

Evaluation of Drought Tolerance in Camelina (Camelina Sativa) Doubled Haploid Lines Using Selection Index Ideal Genotype (SIIG)